Identifikasi Senyawa Metabolit Sekunder dan Analisis Total Fenolik Ekstrak Metanol Daun Sacha Inchi (Plukenetia volubilis linneo) yang Tumbuh di Gorontalo
DOI:
https://doi.org/10.61132/jupenkifb.v1i3.327Keywords:
Folin-Ciocalteu, Phenolics, Plukenetia volubilis LinnaeusAbstract
Sacha inchi (Plukenetia volubilis Linneo) is a plant from the Euphorbiaceae family known for its green star-shaped fruit and brown seeds. This plant has high nutritional value and significant antioxidant activity (Nuha and Sriwidodo 2022). This study aims to identify secondary metabolite compounds and determine the total phenolic content of the methanol extract of Plukenetia volubilis Linneo leaves. The samples were extracted using the maceration method, then phytochemical tests and total phenolic content analysis were carried out using the Folin-Ciocalteu method (Sari et al. 2021). The results of the phytochemical test showed that the methanol extract of sacha inchi leaves contained secondary metabolite compounds such as alkaloids, flavonoids, saponins, and tannins. The total phenolic content obtained from the extract was 19.94%, which indicates the potential for high antioxidant activity. The results of this study indicate that sacha inchi leaves have the potential to be developed as a source of natural bioactive compounds that are beneficial to health.
References
Aribowo, A., dkk. (2021). Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). London: Chapman and Hall.
Bayani, F. (2016). Analisis fenol total dan uji aktivitas antioksidan dari ekstrak buah sentul (Sandoricum koetjape Merr). Hydrogen: Jurnal Kependidikan Kimia, 4(1), 55. https://doi.org/10.33394/hjkk.v4i1.47
Ditjen POM. (2000). Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan RI.
Hanani, E. (2015). Analisis fitokimia. Jakarta: Penerbit Buku Kedokteran EGC.
Harborne, J. B. (1987). Metode fitokimia: Penuntun cara modern menganalisis tumbuhan. Bandung: Penerbit ITB.
Karamać, M. (2009). Chelation of cupric ions by tannin constituents of selected edible nuts. Polish Journal of Food and Nutrition Sciences, 59(1), 55–59.
Kittibunchakul, S., Hudthagosol, C., Sanporkha, P., Sapwarobol, S., Suttisansanee, U., & Sahasakul, Y. (2022). Effects of maturity and thermal treatment on phenolic profiles and in-vitro health-related properties of sacha inchi leaves. Plants, 11(11), 1515. https://doi.org/10.3390/plants11111515
Mosquira, O. M., Correa, Y. M., Buitrago, D. C., & Naranjo, J. (2007). Antioxidant activity of twenty five plants from Colombian biodiversity. Memórias do Instituto Oswaldo Cruz.
Ningrum, A. S., & Halimah, E. (2022). Narrative review: Kandungan kimia dan aktivitas farmakologi tanaman sacha inchi (Plukenetia volubilis L.). Farmaka, 20(3), 112–122.
Nuha, Q. A. L. U., & Sriwidodo. (2022). Sistematika review aktivitas antioksidan tanaman sacha inchi (Plukenetia volubilis L.). Farmaka, 20(3), 104–111.
Nurhayati, B., Salimi, Y. K., & Ahmad, S. A. (2019). Penentuan kandungan fenolik total dan aktivitas antioksidan dari rambut jagung (Zea mays L.) yang tumbuh di daerah Gorontalo. Sustainability (Switzerland), 11(1), 1–14.
Nurjannah, I., Ayu, B., Mustariani, A., & Suryani, N. (2022). Skrining fitokimia dan uji antibakteri ekstrak kombinasi daun jeruk purut (Citrus hystrix) dan kelor (Moringa oleifera L.). Spin: Jurnal Kimia & Pendidikan Kimia.
Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(6), 1142–1145.
Puspa, O. E., Syahbanu, I., & Wibowo, M. A. (2017). Uji fitokimia dan toksisitas minyak atsiri daun pala (Myristica fragrans Houtt) dari Pulau Lemukutan. Jurnal Kimia Khatulistiwa, 6(2), 1–6.
Sari, N. M., dkk. (2021). Aktivitas antioksidan, kandungan total fenolik dan kandungan total flavonoid pada beberapa tumbuhan berkhasiat obat di Kalimantan Timur, Indonesia. ULIN: Jurnal Hutan Tropis, 5(2), 145. https://doi.org/10.32522/ujht.v5i2.6224
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
Waterhouse, A. (1999). Folin–Ciocalteu micro method for total phenol in wine. Department of Viticulture & Enology, University of California, Davis, 152–178.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Jurnal Pendidikan Kimia, Fisika dan Biologi

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.